Journal: Scientific Reports

Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

doi: 10.1038/s41598-025-97729-2

Figure Lengend Snippet: Antibodies used in Immunofluorescence staining.

Article Snippet: MMP-9 , Rabbit polyclonal , 20ug/ml , Novus , NBP2-41233.

Techniques: Immunofluorescence, Staining, Concentration Assay

Journal: Scientific Reports

Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

doi: 10.1038/s41598-025-97729-2

Figure Lengend Snippet: Antibodies used in Western blot.

Article Snippet: MMP-9 , Rabbit polyclonal , 20ug/ml , Novus , NBP2-41233.

Techniques: Western Blot, Concentration Assay

Journal: Nutrients

Article Title: Ginsenoside Rh4 Suppresses Metastasis of Esophageal Cancer and Expression of c-Myc via Targeting the Wnt/β-Catenin Signaling Pathway

doi: 10.3390/nu14153042

Figure Lengend Snippet: ( A ) Migration and invasion images of KYSE30, KYSE150, and KYSE410 cells. ( B ) Counting cells after crystal violet staining ( C , D ) Protein level of E-cadherin, N-cadherin, Vimentin, snail, MMP2, and MMP9 in KYSE30, KYSE150, and KYSE410 after Rh4 treatment (10 and 20 µM). All data are presented as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Rabbit antibodies against E-cadherin, snail, MMP2, MMP9, Wnt, β-catenin, and c-Myc and mouse antibodies against N-cadherin and Vimentin were obtained from Proteintech Group, Inc (Chicago, MI, USA).

Techniques: Migration, Staining

Journal: Nutrients

Article Title: Ginsenoside Rh4 Suppresses Metastasis of Esophageal Cancer and Expression of c-Myc via Targeting the Wnt/β-Catenin Signaling Pathway

doi: 10.3390/nu14153042

Figure Lengend Snippet: ( A , B ) Protein level of E-cadherin, N-cadherin, Vimentin, MMP2, MMP9, Wnt, β-catenin, and c-Myc in the lymph node. ( C ) Body weight change curve of six groups of mice. ( D ) Representative image of KYSE30 popliteal lymph nodes of measured groups. ( E ) Popliteal lymph nodes were measured after sacrifice. All data are presented as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Rabbit antibodies against E-cadherin, snail, MMP2, MMP9, Wnt, β-catenin, and c-Myc and mouse antibodies against N-cadherin and Vimentin were obtained from Proteintech Group, Inc (Chicago, MI, USA).

Techniques:

Journal: BMC Cancer

Article Title: DOCK1 regulates the malignant biological behavior of endometrial cancer through c-Raf/ERK pathway

doi: 10.1186/s12885-024-12030-1

Figure Lengend Snippet: DOCK1 modulated the expression of related proteins and signaling pathway of endometrial cancer cells. ( A ) The expression of E-cadherin, MMP9, Ezrin and Bcl-2 protein was analyzed after DOCK1 knockout or overexpression. ( B ) The effect of DOCK1 knockout or overexpression on c-Raf/ERK signaling pathway related protein

Article Snippet: The primary antibodies (1:1000) were shown as follows: rabbit anti-Bcl2 antibody (Boster, China), rabbit anti-DOCK1 antibody (CST, USA), rabbit anti-E-cadherin antibody (Proteintech, USA), rabbit anti-Ezrin antibody (Proteintech, USA), mouse anti-GAPDH antibody (CST, USA), rabbit anti-MMP9 antibody (CST, USA), rabbit anti-tubulin antibody (ABclonal, China), rabbit anti-phospho-C-Raf (S338) antibody (CST, USA), rabbit anti-C-Raf antibody (CST, USA), rabbit anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (CST, USA), rabbit anti-ERK1/2 antibody (CST, USA).

Techniques: Expressing, Knock-Out, Over Expression

Journal: BMC Cancer

Article Title: DOCK1 regulates the malignant biological behavior of endometrial cancer through c-Raf/ERK pathway

doi: 10.1186/s12885-024-12030-1

Figure Lengend Snippet: The inhibition of DOCK1 attenuated the growth of xenograft tumors in vivo. ( A-B ) The images of nude mice and subcutaneous tumors at 4 weeks after injecting DOCK1-KO Ishikawa cells. ( C-D ) The growth and weights of tumors were analyzed in DOCK1-KO group and control group. ( E ) The expression of E-cadherin, MMP9, Ezrin and Bcl-2 protein on xenograft tumors by immunohistochemical assay. * P < 0.05

Article Snippet: The primary antibodies (1:1000) were shown as follows: rabbit anti-Bcl2 antibody (Boster, China), rabbit anti-DOCK1 antibody (CST, USA), rabbit anti-E-cadherin antibody (Proteintech, USA), rabbit anti-Ezrin antibody (Proteintech, USA), mouse anti-GAPDH antibody (CST, USA), rabbit anti-MMP9 antibody (CST, USA), rabbit anti-tubulin antibody (ABclonal, China), rabbit anti-phospho-C-Raf (S338) antibody (CST, USA), rabbit anti-C-Raf antibody (CST, USA), rabbit anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (CST, USA), rabbit anti-ERK1/2 antibody (CST, USA).

Techniques: Inhibition, In Vivo, Control, Expressing, Immunohistochemical staining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: LPCAT1 promotes brain metastasis of lung adenocarcinoma by up-regulating PI3K/AKT/MYC pathway

doi: 10.1186/s13046-019-1092-4

Figure Lengend Snippet: Knockdown of LPCAT1 inhibited the proliferation, migration and invasion of NSCLC cells. a, b PCR and Western blotting showed the expression of LPCAT1 in HCC827 and PC-9 cells stably transfected with shNC and shLPCAT1. c The proliferative ability of HCC827 and PC-9 cells after transfection was evaluated by CCK-8 assay. d GSEA analysis showed that the LPCAT1 amplification status was positively correlated with genes related to “KEGG CELL CYCLE” and “GO CELL CYCLE G1-S TRANSITION”. e , f The cell cycle was flow cytometrcally studied after PI staining, and the data were processed with ModFit LT program. Down-regulation of LPCAT1 induced G1 phase arrest. g Wound-healing assays were performed to assess NSCLC cells migration. Wound closure was determined 24 h after the scratch. h , i Representative images and quantification of transwell assays indicated the migration and invasive capability of NSCLC cells stably transfected with shNC and shLPCAT1. j The expression of MMP-9 was determined by Western blotting. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, t test

Article Snippet: The antibodies used were as follows: LPCAT1 (16112–1-AP, ProteinTech, Chicago, IL, USA), MYC (10828–1-AP, Proteintech), GAPDH (60004–1-Ig, Proteintech), AKT (4691, Cell Signaling Technology, MA, USA), p-AKT (4060, Cell Signaling Technology), PI3K (4249, Cell Signaling Technology), MMP-9 (10375–2-AP, Proteintech).

Techniques: Migration, Western Blot, Expressing, Stable Transfection, Transfection, CCK-8 Assay, Amplification, Staining